![]() Since then, the autophagic pathway has been studied extensively during the metamorphosis of many other lepidopterans. For example, the term “autophagic cell death” was introduced while studying metamorphic degeneration of muscle tissue in silkmoths ( Lockshin and Williams 1965). sexta is a well characterized model system for studying programmed cell death and metamorphosis ( Jones et al. 1992 Truman and Reiss 1995 Wieczorek et al. sexta, NCBI: txid7130) is a key model for studying biochemical mechanisms in insect biochemistry, physiology, neurobiology, development, and immunity ( Kay et al. sexta as a model organism.Īs a large, easily grown insect, the tobacco hornworm ( M. ![]() sexta genome improves resequencing studies and benefits future research on M. We demonstrate that improved continuity of the M. The new reference assembly is accessible in annotated form for public use. We annotated the assembly by lifting over existing annotations and supplementing with additional supporting RNA-based data for a total of 25,256 genes. The assembly is 470 Mb and is ∼20× more continuous than the original assembly, with scaffold N50 > 14 Mb. sexta, JHU_Msex_v1.0, applying a combination of modern technologies in a de novo assembly to increase continuity, accuracy, and completeness. However, current studies rely on the highly fragmented reference genome Msex_1.0, which was created using now-outdated technologies and is hindered by a variety of deficiencies and inaccuracies. The tobacco hornworm, Manduca sexta, is a lepidopteran insect that is used extensively as a model system for studying insect biology, development, neuroscience, and immunity. ![]()
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